molecular devices 96 well microplate reader. book Page 9 Wednesday, July 7, 2010 2:09 PM. molecular devices 96 well microplate reader

 
book Page 9 Wednesday, July 7, 2010 2:09 PMmolecular devices 96 well microplate reader  Both readers were heated to 37ºC prior to setting up the assay

4 nm Microplate Capacity Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates Incubation Temperature control range from Ambient +4º to 50ºC Temperature variation ± 0. #T-0254) • Lysozyme (Sigma cat. Molecular Devices i3x. g. The system provides automation for up to 50 microplates for easy walkaway automation. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 1 FP • Fluorescence Polarization detection for fluorescein- or . g. For more sample throughput, both readers can be easily integrated into full roboticVisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath: Datapaq Express (Vial Rack Bottom Label 2D Reader). Open the catalog to page 5. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. #655075 and #781075) SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax Injector Cartridge *Figure 1. 96, 384, 24, 12, 6 well types. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. #M5e) Methods Microplate reader compatibility test. iD5. 0 500 1000 1500 2000 2500 3000 3500 time (sec. The Molecular Devices Versamax Microplate Reader INCLUDED:. Microplate readers can be either single-function instruments or “multimode. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Brand: MOLECULAR DEVICES. If the best wavelengths are unknown, the Gemini EM reader will find. 0. 2 OD at 200 nm; this decreased to 0. 0200-6263. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. Monochromator, tunable in 1-nm increments. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. This reader provides visible. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. For more sample throughput, the system canRating: 3. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. The color touch screen provides a visual user interface, making programming fast and intuitive. Taking into account the price differences between each seller and each country. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). 40266. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Samples were run in triplicate. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. We would like to show you a description here but the site won’t allow us. 39. The procedure enables direct translation of any published equation. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Retrieving. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. Category 2 and 3 microplate readers use gradient heating as well. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. (FP) configured microplate readers. 48 W x 12. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. field-upgradeable option with one-of-a-kind brightfield cell segmentation, green and red. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. New SpectraMax iD5 Multi-mode Microplate Reader. Detergent Compatible Bradford assay. Get. SoftMax Pro Software allows you to set up and run a complete protocol for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. white 96 well plate and 75. 19 H mm that meet microplate industry standards. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. ©2010 molecular Devices, inc. For more great deals on Microplate readers by Molecular Devices, please browse our Molecular Devices Microplate readers page. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 5 mm. 76 L x 85. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Instrument settings can be saved as a protocol file and used repeatedly for reading differentThe Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. 030 fmol Fluorescence Polarization (FP). personalized as your research. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. Consult Molecular Devices's entire Threshold Immunoassay System catalogue on MedicalExpo. 1/17 1907B Printed in USA . #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. 05. SmartReader 96: Designed for optimal ELISA Testing. Bioz Stars score: 86/100, based on 1 PubMed citations. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. In . TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. absorbance, fluorescence, and luminescence with the added functionality of. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. No accessories are needed and SoftMax® Pro Software. Setup Guide. Multimode Readers Comparison Chart. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. SOFTmax PRO allows you to select excitation and emission values from the literature and simply input their values for the monochromator settings. Bioz Stars score: 86/100, based on 1 PubMed citations. Top or bottom detection is available with a simple click of a button. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. Five-second read per plate. This is equivalent to having 511 built-in filters,Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. ©2017 Molecular Devices, LLC . The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. Paradigm: Delta mP. 5. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. SpectraMax 250 User. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. 1. For more sample throughput, both readers can be easily integrated into full robotic Microplate Readers. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. 50 racks/case for 384-well (16 channel pipettor head. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. a white 96-well half-area microplate (Corning cat. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Infinite 200 Pro Microplate Reader. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. reagent basin for ease of pipetting into. AquaMax 4000 Microplate Washer (Molecular Devices Cat. We would like to show you a description here but the site won’t allow us. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. added to 96-well at 37 °C for 2 h, then the absorbance was measured at 450 nm by a. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. 340‚ 850 nm. The plate reader optics are tunable for. Fluorescent DNA quantitation. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. When the user applies a set temperature of 37°C, the top is heated to 37. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a black, clearbottom microplate. The kinetic top-read mode of a SpectraMax M3 microplate reader (Molecular Devices, CA, USA). Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. Comparing Luminescence sensitivity of SpectraMax Microplate Readers using ATP Assay | Molecular Devices Author: Molecular DevicesMake the most of your budget with the VersaMax™ ELISA Microplate Reader, our affordable, visible spectrum absorbance reader for 96-well microplates. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplatethe iD5 reader’s filter system. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 3:40 . ValitaTiter Plus 96-well (1 plate) VAL004. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Or scan up to 384 wells at any time to determine the best assay conditions. 8. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aSpectraMax Microplate Readers. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. We would like to show you a description here but the site won’t allow us. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. SpectraMax® M3/M4/M5/M5e, GeminiTM EM/XPS, FlexStation® 3. Step 3: Click “Settings” and a screen will appear (Figure 1). It also integrates with the. Well scanning With well scanning, multiple points within each well can be read, providing a high. #ID3-STD) L-Tryptophan (Sigma cat. No filter change with dual monochromator optics. /well integration in 384-well luminescence mode Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. 63. Standard SBS 96 and 384 well plates (128. Fluorescence Microplate Reader Comparison Chart. 384 well polystyrene microplates have plate dimensions of 127. ’s GloMax®-96, for instance, is a dedicated luminometer, whereas Bio-Rad Laboratories’ iMark and xMark strictly measure absorbance. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Plate reader instructions Computer. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Timothy Bolus, MPM, MBA, Compliance Program Manager. Published Dec 16, 2020 | Updated Dec 16, 2020. The screw remains inside the instrument. For more sample throughput, the system can well. The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Use black microplates. Features. Less background noise gives a better limit of detection and so a relative increase in usable signal. 3% in white 96- and. Endpoint, kinetic, spectrum. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. A multitude of modalities. 63. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. microplate readers. A microplate reader as . 69: 85. 98 Bovine serum albumin mg/mL O. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Avoid losing high signal. Plate wash time (300 µl). *Simple analysis and optimization. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. to each well. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. Average StDev (mP) 1. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. An example of dual-luciferase assay results. in space. With optimized reagents, option to operate the. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. 2 OD at 200 nm. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Molecular Devices VersaMax Microplate Reader Specifications. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesThe SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Transfer of an assay to 96-well microplate technology increases throughput significantly. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. This unlocks the transport slide. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all leading partner robots. It features a modular design that allows users to add or change detection cartridges according to their needs. section, select plate section titled. All stand-alone instrument functions can be controlled using the software. Blank wells containing 50 µL culture medium only (n = 8) were also prepared. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. The reader adds up to three transfers of assay reagents from a 96- or 384. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. molecular devices gmbh tel. Molecular Devices is a leader in comprehensive compliance solutions with microplate detection systems. Flexible for 6-well to 384-well microplates and cuvettes. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit (signal 3X SD of baseline) 8. +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan molecular devices Japan, osaka. 2 2601A ValitaTiter. Jon Cuccui, Brendan W. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. VANTAstar™ Flexible Multi-mode Microplate Reader. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Integrated stacker option. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the. This reader's dual monochromators for. fluorescence microplate reader from Molecular Devices. The iMark. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. The OptiMax by Molecular Devices is a tunable microplate reader. 1. SoftMax Pro Software’s. 2 nm Wavelength Bandpass 2. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. i3x Multi-Mode Microplate Reader. book Page 9 Wednesday, July 7, 2010 2:09 PM. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 76 L x 85. It is a UV/Vis spectrophotometer/microwell reader with built-in cuvette port and microplate drawer. 6:30 . ValitaTiter 96-well (Pack of 20). 2 2600A ValitaTiter. Fluorescence Microplate Reader Comparison Chart. A. 96-well microplate at 200 µL/well. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. 2. SpectraMax® Microplate Reader Suite of Products Knowledge Base. #SpectraMax L Config) Methods. Description. SpectraMax 250 User. Biochemical characterization was conducted, using GEN III MicroPlate™ Biolog identification system and confirmed with molecular identification via 16S rDNA gene. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Type: Microplate Reader. Running both standard spectrophotometer and microplate. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 0. Molecular Devices gmbH Tel. validation, maintenance, and repair documentation. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Detection by Low noise PMT with simultaneous digital and analog modes. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. #PLUS 384) Methods. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. Related Products: Low Binding Plates. These instruments are all elegant and simple in their form and function. Important things to consider when purchasing a multimode microplate reader. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Description. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. 300 µL of Detergent Compatible Bradford reagent was added to each well. 1 sec. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. Making it possible to perform kinetic analysis of solid. ± 0. M3-M5e_manual. For biochemical. Triplicates of each BSA concentration were made for this assay plate. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. the appropriate well of the microplate. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. for ValitaTiter Plus standards read on Molecular Devices readers. 3. BMG LABTECH. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. Step 1: Turn on your microplate reader. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). 5 mm and 15. Dynamic range > 9 orders of magnitude. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Microplate Readers. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Incubate the plate at 37 °C for approximately one hour. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 2 W x 44. A 1:10 serial dilution of ATP was prepared in medium. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. With theThe StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. 03 pM. Operates as a stand-alone reader or with input/output stackers and bottom reading optics. Multi-Mode Microplate Reader for . System setup and calibration are controlled from within SoftMax Pro Software. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 2 OD at 200 nm; this decreased to 0. Optical System: High energy xenon flash lamp. Find a new or used microplate reader and lab equipment for sale and auction at LabX. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. Plate Formats: 6 to 384-well microplates. 125 pM. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. The CellTiter-Glo assay from Promega uses luciferase. The system provides options for 20-, 40-,. Microplate Reader, Molecular Devices Optimax, Refurbished. In addition, the iD5 reader measures TRF and FP and. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Microplate luminescent reader assay for ERK activity. the appropriate well of the microplate. In addition, SoftMax Pro SoftwareUsing Molecular Devices microplate readers and SoftMax. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. Molecular Devices M5 Plate Reader Plate Calibration Form . Molecular Devices SpectraMax 384 Plus Microplate Reader. These robust readers have been placed in labs from. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. NanoRacks added it to their line of commercial . 04 sec. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. University of Reading Simon Lydford: Molecular Devices (UK) Ltd. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. 0 96 Well Plate. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 012 fmol 0. 0 500 1000 1500 2000 2500 3000 3500 time (sec. 2 Pages.